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Frequently Asked Questions

How much sample is required to get a DNA profile?

Frequently asked questions
The DNA profile obtained depends on the integrity of the sample. If the DNA in the sample is degraded or insufficient then you may not be able to generate a DNA profile. Y STRS's is used when you have a mixture of male and female DNA (typically from a vaginal swab). It is able to detect the male portion of the sample.
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What was the purpose of storing a dog's DNA sample after recording the dog's DNA profile?

Australian Shepherd Club of America, inc | DNA FAQ
The initial reason for storing blood samples was in the event that ISAG came up with a set of universal markers that were used by all labs for canine DNA parentage verification programs. Since the labs used by other registries (i.e. AKC, UKC) did not adopt these, ASCAs lab decided to stay the course with the markers that had been used successfully for a number of years for our breed.
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My dog already had a DNA profile done with the AKC. Can that sample be used?

CHIC DNA Clinic: Breed Information: Kerry Health: United Sta...
No. There is a clear distinction between samples provided to the AKC which are used for parentage verification and overall stud book integrity versus samples provided to the bank for research purposes. Samples may be collected at the same time however, and sent independently.
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How much of an evidence sample is needed to produce a DNA profile?

Reliagene
This depends on condition of the sample and the type of testing performed. ReliaGene specializes in difficult forensic samples, and has achieved results from minute quantities, rootless hair shafts, human remains over 150 years old, and challenging male/female "mixtures." Contact ReliaGene's forensic department to discuss the specifics of your case.
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How is the DNA sample obtained?

DNA FAQs
A painless swabbing of the inside of the cheek with a sterile cotton swab is used for our laboratory samples.
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What is a DNA Profile?

Australian Shepherd Club of America, inc | DNA FAQ
To prepare a DNA Profile, multiple different genetic locations are identified in your dog. If, for example, the first location identified on a dog has genes labeled A and B, that means that the dog inherited an A gene from one parent and a B gene from the other. When the parents are tested, that exact same location will be examined. To continue this hypothetical example, assume the Sire is AJ and the dam is BK.
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I sent my DNA sample to AKC but haven’t heard anything. What is the status of my DNA profile?

American Kennel Club - Registration Requirements
Breeders/owners fulfill the requirement when the DNA test kit arrives at DNA Operations in Raleigh and is logged into the Database, even though an additional six to eight weeks are required to obtain the actual DNA profile. Any litters that are pending because we need a DNA test for the sire will be released when we receive the sample.
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How much DNA is required?

CIDR - FAQs
CIDR requires 20 µg of DNA per sample. Prior to initiating the whole genome scan, all samples will be run with several test markers to ensure acceptable DNA quality and quantity.
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What do I do with the DNA FilerTM kit after I collect the sample?

DNA Filer FAQ
File it! The purpose of using the DNA FilerTM kit is to insure that a sample of your DNA is available in the future, even if you are not available in person. Your kit can literally be stored in your filing cabinet or safety deposit box, along with any background material or instructions you wish to provide yourself. Depending on your goals, you may want to include such items as physical description and identifying marks, personal and family medical history, or genealogy charts.
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Can I submit an anonymous DNA sample?

FAQs - Instant MD Paternity DNA Testing
Test samples may be submitted anonymously to protect your identity — simply change or omit names and send payment in the form of a cashier's check.
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Where do I need to go to have my DNA sample collected?

Parentage FAQs & Definitions
Our customer service representatives will assist you in setting up a convenient appointment to have your samples collected at a collection site near you. American Association of Blood Banks (AABB): is an international, not-for-profit association dedicated to the advancement of science and the practice of transfusion medicine and related biological therapies (this includes paternity).
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How do I provide a sample for DNA analysis?

Maxxam Analytics - The DNA Lab
You may provide a DNA sample by scheduling an appointment at a certified collection site. When you arrive for your scheduled appointment, a qualified technician will collect a DNA sample through what is known as a buccal swab method. Collection is simply done by gently rubbing a swab inside the mouth for a short period of time. This is a painless and routine procedure.
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Will the DNA sample be safe?

International Biosciences - Frequently Asked Questions About...
Yes. The DNA sample collection kits we will supply you with are tamper proof and all results are stored securely.
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Why should I keep a DNA sample of my child?

A photograph and description is required immediately when a child, or any individual, is lost or missing. That's why KidSURE™ Safety Net is such a valuable tool to investigators. However, if the individual is not located right away and an ongoing investigation ensues, DNA technology can play a vital role in recovery. DNA represents the evolution of trace identification. It is permanent and far more accurate than fingerprinting.
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What is DNA?

WorldFamilies.net: Where Genealogy meets DNA testing
DNA or DeoxyriboNucleic Acid is the molecule sometimes known as the blueprint of life. It contains the genetic code that exists in each cell of our bodies and is found throughout nature in living things. Genealogists can compare certain sequences or markers on specific chromosomes between living individuals in hopes of finding common ancestors.
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What can a DNA Identification Profile be used for?

International Biosciences - Frequently Asked Questions About...
To safeguard the rightful heirs and ensure that they will inherit your estate through the exclusion of false claims on your inheritance. a precaution for any possible future identification/verification requirements for those who work in high risk jobs e.g. in cases of kidnapping, accidental death etc.
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What type of tumor sample is required for Oncotype DX?

Oncotype DX: Frequently Asked Questions (FAQ) - Genomic Heal...
Oncotype DX is performed using formalin-fixed, paraffin-embedded invasive breast tumor tissue obtained by lumpectomy, mastectomy, or core biopsy.
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What amount of sample is required?

Proteomics International - Frequently Asked Questions
mg total protein (assuming a complex protein mixture) is required for a large format (20cm) gel stained with Coomassie. mg total protein (assuming a complex protein mixture) is required for a small format (12cm) gel stained with Coomassie. Other stains are possible which require smaller amounts of protein, but their use depends on the desired outcome. A guide to prices is on this website, however, please note the final price will depend on the number of gels required.
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Q6. I have DNA sample in TE buffer. Can you sequence it or do I have to repurify it in water?

Sample Preparation Frequently Asked Questions
We normally recommend eluting the DNA in water. However, we can still sequence your DNA in TE buffer using our modified reaction protocol. Please let us know in advance.
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What does the lab do with the actual DNA sample once the test is completed?

Family Tree DNA - we do genetic tests for your genealogy que...
The DNA extracted from your sample is kept by the lab for a period of 25 years for your exclusive use. It is important to note that Family Tree DNA adopts a "double safety net" in regard to your sample: Arizona Research Labs at the campus of the University of Arizona keeps the DNA associated to a number and not a name, while Family Tree DNA offices keep the database with no access to the actual sample.
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How do I collect a DNA sample?

American Kennel Club - Registration Requirements
A small bristle brush is inserted in the dog's mouth, swirled against the inside of the cheek, and the sample is collected. It is this simple collection process that makes it possible to use DNA on a large scale to help the accuracy of the AKC registry. AKC has two types of kits. The regular kit is available at no charge, and the $40 processing fee (per dog) is due when the sample is returned to the AKC. We also offer a Prepaid Test Kit for only $35 (per dog).
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Where do I send my dogs DNA sample once it has been collected?

Australian Shepherd Club of America, inc | DNA FAQ
The collected sample should be sent directly to ASCAs approved lab along with the accompanying paperwork. The instructions you receive with your kit will provide details on collection of the sample and the address of our lab. DO NOT SEND THE DNA SAMPLE TO THE ASCA BUSINESS OFFICE. This will only delay your DNA profile and Certificate being mailed to you. If you want to know if the lab received your sample, please send it return receipt.
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Can I combine more than one DNA sample on a single canvas?

DNA11 - From Life Comes Art
Yes. We have an art piece that is designed specifically to allow you to combine the DNA of two, three, or even four people on a single canvas. We call it “SplitScreen”. It’s perfect for couples, family members, business partners, and friends. You can learn more about it here.
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Is there a minimum age for collecting a DNA sample?

FAQ - How to Collect a DNA Test Sample
No. Because DNA is set at conception and generally does not change, a paternity test can be performed on a person of any age-even on a sample from an unborn child (through prenatal testing). At birth, blood from the umbilical cord can be taken for use as a DNA sample source, or a buccal swab can be collected from the newborn.
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Q5: How much DNA is required for sequencing?

Stony Brook DNA Sequencing Facility
If possible, quantitate the amount of purified DNA by measuring the absorbance at 260 nm or by some other method. In general, higher DNA quantities give higher signal intensities, however, if the quantities above are exceeded problem will occur with sequencing. The template quantities stated above should work with all primers. You may be able to use even less DNA, especially when sequencing with the -21 M13 primer.
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What is the CSS PROFILE and is it required?

Linfield Facts: FAQ
Students who apply under the Early Action deadline may choose to submit the CSS PROFILE. It is used to determine an estimate of need-based aid in January. The CSS PROFILE is not required and students who submit the PROFILE form by November 15 must file the Free Application for Federal Student Aid by February 1 to receive any need-based aid.
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