Back to top Can I use HEPES buffer in my cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: HEPES and other organic buffers can be used effectively with many cell lines (see Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130: 305). However, be aware that the compound can be toxic, especially for some differentiated cell types, so its effects should be evaluated before routine use (People, C.A., et al., (1982) In Vitro 18: 755). HEPES has also been shown to greatly increase the sensitivity of media to the phototoxic effects induced by exposure to fluorescent light. [Zigler, J.S.
Related QuestionsBack to top What should the osmolality of my cell culture medium be?
ATCC: Frequently Asked QuestionsAnswer: Most established vertebrate lines will tolerate a rather large variation in osmotic pressure. The useful range of osmolality of cell culture media for vertebrate lines is between 260 to 320 mOSM/kg. Invertebrate lines vary greatly in their sensitivity to osmotic pressure. For example, the snail embryo ATCC CRL-1494 requires medium of about 155 mOSM/kg, while some insect media prefer 360 to 375 mOSM/kg.
Related QuestionsBack to top Can antibiotics and/or antimycotic agents be added to the cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: Most cell culture technologists avoid using antibiotics for routine culture work. Antibiotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks (e.g.
Related QuestionsWhy are some cell culture media better than others?
Welcome to Osmolality.com | Osmolality | FAQMedia originally used for growth of mammalian cells were based on biological fluids, such as plasma. This type of media suffered many disadvantages including batch variation and vulnerability to contamination.
Related QuestionsHow can cell culture media be made?
Welcome to Osmolality.com | Osmolality | FAQA bioreactor allows the growth of human tissues outside of the body that amass and behave like those in the body. Another is the Rotary Cell Culture System, as it provides a gentler environment than a dynamic or static tissue culture system, thereby allowing cells to aggregate, grow 3-dimensionally, and differentiate. The result will be cells or tissues that closely resemble the in vivo tissue equivalent.
Related QuestionsWhat if tissue culture medium is unavailable?
FAQ 3Samples for chromosomal analysis can still be taken. We suggest drawing maternal blood in a sterile tube, spinning it down and using the plasma or serum as transport medium for the fetal tissue samples. Studies suggest that this is considerably more successful than the use of sterile saline. If maternal serum can not be obtained, one can use sterile saline for shipment.
Related QuestionsBack to top How do I change the medium in (feed) a suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Suspension cell lines can be fed by the simple addition of fresh medium to the culture (if room is available) or by separating the cells from the old medium by centrifugation (125 x g for 5 min) with subsequent resuspension of the cell pellet in fresh medium. However, with most suspension cell lines, a simple addition of medium is the preferred method. In either case it is absolutely necessary to feed the cultures before the cells reach their maximum saturation density.
Related QuestionsWhat is a buffer?
Acid ReDux Acid Reflux SupplementA buffer is a solution containing salts of weak base that is capable of neutralizing both acids and bases and acts to maintain the pH of a solution. In other words, a buffered solution contains both a weak acid (e.g., acetic acid) and its conjugate weak base (e.g., sodium acetate) and its pH changes only slightly upon the addition of acid or base. The weak acid acts as a buffer when base is added to the solution, and the weak base acts as a buffer when acid is added to the solution.
Related QuestionsFAQBuffer raises pressure significantly and is never to be used unless called for in a recipe. Use only the specific type called for – never interchange Buffer types. Precision's Spherical Buffer greatly improves pattern density and pattern core when used with prescribed loads. Never use buffer unless you have data requiring same from a reputable source and then only the buffer type specified in the loading data.Related Questions
Voodoo Lab - Pedal Switcher & Commander FAQNot if you don't want to! The buffer is a completely separate circuit from the loops. You can choose to use the ultra-transparent input buffer, or just patch directly into the first loop. You might want to experiment with which sounds best in your rig.Related Questions
Do you sell culture medium and supplies?
FAQ From New York WormsNo. We feel that it is easier for our customers to use our started cultures rather than attempt to start their own. (See next question) Precisely the reason that we don't sell culture supplies. Unless one is extremely careful, wild "common" fruitflies can contaminate the stock, producing all flying fruitflies. The only remedy is to dispose of everything and start over.
Related QuestionsShould I use a light, medium, or dark formula?
Frequently Asked Questions - Sunless.comAlways start with a light or medium formula when you try a new brand. Light/medium/dark varies considerably from product to product. In general, the department store brand dark is heavier than the drugstore brand dark. And don't forget that two applications of a light or medium formula may give you better results than one application of dark.
Related QuestionsWhat medium will we use?
Artista Creative Safari's for WomenFor a variety of reasons, we use acrylic paint and gesso on paper, wood, and stretched canvas. We've noticed more and more contemporary artists are utilizing acrylics for good reason- they can be used for a wide range of techniques from thin washes like watercolor to layered, thick textures similar to oils. They quickly dry and unlike oils, are low odor and easily thin and clean with water.
Related QuestionsPet Portraits of your cat, dog or any other animal by Jeffre...I use acrylic paint on quality pre-stretched canvases. I prefer painting on the thicker (1 ½ inch) canvases if the artwork hangs on the wall without a frame. This is how the artwork hangs on my wall and it looks great. The thinner (3/4 inch) canvases are easily framed, and the choice is up to my clients’ preferences.Related Questions
Back to top Why do some cell lines require sodium pyruvate? How much should I add to the medium?
ATCC: Frequently Asked QuestionsAnswer: Pyruvate is an intermediary organic acid metabolite in glycolysis and the first of the Embden Myerhoff pathway that can pass readily into or out of the cell. Thus, its addition to tissue culture medium provides both an energy source and a carbon skeleton for anabolic processes.
Related QuestionsDo you use a medium to create thick brushwork?
FAQSusan and I use mineral spirits to clean our brushes and to thin our paint down a bit for initial washes, but just use the paint straight without any medium for the rest of the painting. The way I get such thick brushwork simply has to do with laying out lots of paint and really loading up my brush. I will even mix up large piles of the various colors I'm using in a painting with my palette knife so I will have plenty of paint to work thickly with.
Related QuestionsBack to top How can I adapt a monolayer cell line to grow in suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Not all cell lines can be adapted to suspension growth. In general, normal diploid anchorage-dependent (must be attached to a substrate to grow) cells cannot be adapted without the use of microcarrier beads to which they can attach. Lines such as L-929 (ATCC CCL-1) and HeLa (ATCC CCL-2) which are not anchorage-dependent can be adapted and variants that grow in suspension already exist.
Related QuestionsBack to top What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?
ATCC: Frequently Asked QuestionsAnswer: While the levels of carbon dioxide in cell culture systems vary from that in ambient air (about 0.03%) up to 40% in air, generally either no added CO2 or 5% to 10% CO2 in air are the most frequently used. It is very important to adjust the concentration of sodium bicarbonate used in a medium to that required for equilibration with the level of CO2 used in the gas phase. Cells in culture produce CO2 and require small amounts of the compound for growth and survival.
Related QuestionsBack to top Why is it important to limit exposure of cell culture media to fluorescent lights?
ATCC: Frequently Asked QuestionsAnswer: An important but often overlooked source of chemical contamination results from the exposure of media containing riboflavin or tryptophan to normal fluorescent lighting (see references below). These media components are photoactivated by UV radiation emitted from most fluorescent lights and give rise to hydrogen peroxide. This generates free radicals that are toxic to cells; the longer the exposure the greater the toxicity.
Related QuestionsIs the glass bottom SensoPlate recommended for cell culture?
Greiner Bio OneWhile this plate provides excellent flatness and clarity for microscopic examination, some cell lines may interact with the adhesive used in production. It is best to pre-test your culture if you plan to incubate them in the SensoPlate for longer than 24 hours.
Related QuestionsWhat medium do you use for the comic? Is it originally black and white?
FAQ: Frequently Asked QuestionsYup. I draw it with my trusty blue pencil (69 cents at your local Loomis & Toles) and ink it with a fineliner. For Episodes 2 & 3 I used a kid's watercolour set for shading. Cheap and fairly effective. For Episodes 1 (the revamp) 4 & 5, I scanned the comic unshaded, and shaded it in Painter Classic (a very nice little program I got with my wacom tablet). Later I moved on to Painter 8, which has a much more finished look than PC.
Related QuestionsWhich brush material do I use for each medium?
Rosemary&Co - Quality Artists' BrushesAs a general rule of thumb the finest hair is used for watercolour: Kolinsky Sable being the ultimate, followed by Pure Red Sable, Squirrel etc. At the moment, there are some excellent Nylon fibres which imitate Sable thus keeping costs low. It's not just about 'snap & spring' which are familiar terms but how the brush strokes the paper. Sable has the advantage of holding a huge quantity of liquid and 'flows' when pressed onto the paper.
Related QuestionsWhat kind of medium do you use in your artwork?
Abstract Art | Chris Quinlan Original Irish Artist | buy pai...Chris Quinlan Artist uses only quality oils and acrylics on stretched canvas. Sometimes Oil pastels are used but only on paper. There are no Oil pastels in the Galleries as of yet. Oil pastels and other mediums can be requested in commissioned artwork. Chris Quinlan Artist does not sell prints, we only sell original artwork. Each painting purchased is a unique piece.
Related QuestionsS.I.G. F.A.Q.I use predominantly watercolors and also ink, gouache, markers, and colored pencils. Sometimes I also use pastels and acrylics.Related Questions
What kind of medium do you use in your work?
The art of Popeye Wong: Frequently Asked QuestionsI work usually with graphite pencil, acrylics (brushed and airbrushed), and more recently I work also in digital media. This is due to the fact that my type of work is commercial, where faster results are needed. However, I like to paint a lot with oils whenever possible. Once in a while I use pastels too.
Related QuestionsWhat Medium/Media you use?
Jade Illustrations - Artist - (Artworks and llustrations by ...I am able to use a couple of mediums, starting with pencils, inks, markers, acrylics, gouache, watercolour, Colour pencils, sometimes I use pastels, and of course my forte is Digital art, such as Photoshop painted, Vector graphics with Corel or freehand.
Related QuestionsDo you use both 35mm and medium format?
Donovan Photographer :. FAQ .: California Wedding Photograph...I have used both medium format and 35mm to cover weddings. I now prefer and use 35mm (digital) cameras. They are smaller, lighter and quicker.
Related QuestionsCan I Use A "C" Cell Rechargeable With My Seiko Cleancut?
Frequently Asked Questions (FAQ)No, you should not use a rechargeable battery with your Seiko Cleancut and here's why. The speed of any DC motor is directly related to the voltage of the power supply; the higher the voltage the faster the motor turns. By their nature rechargeable batteries do not have as high a voltage as alkaline batteries. For instance a fresh alkaline "C" cell, which is a 1.5 volt battery, will test out as high as 1.6 volts right out of the package.
Related QuestionsWhy do scientists want to use stem cell lines?
FAQs [Stem Cell Information]Once a stem cell line is established from a cell in the body, it is essentially immortal, no matter how it was derived. That is, the researcher using the line will not have to go through the rigorous procedure necessary to isolate stem cells again. Once established, a cell line can be grown in the laboratory indefinitely and cells may be frozen for storage or distribution to other researchers.
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