Back to top What should the osmolality of my cell culture medium be?
ATCC: Frequently Asked QuestionsAnswer: Most established vertebrate lines will tolerate a rather large variation in osmotic pressure. The useful range of osmolality of cell culture media for vertebrate lines is between 260 to 320 mOSM/kg. Invertebrate lines vary greatly in their sensitivity to osmotic pressure. For example, the snail embryo ATCC CRL-1494 requires medium of about 155 mOSM/kg, while some insect media prefer 360 to 375 mOSM/kg.
Related QuestionsBack to top Can I use HEPES buffer in my cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: HEPES and other organic buffers can be used effectively with many cell lines (see Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130: 305). However, be aware that the compound can be toxic, especially for some differentiated cell types, so its effects should be evaluated before routine use (People, C.A., et al., (1982) In Vitro 18: 755). HEPES has also been shown to greatly increase the sensitivity of media to the phototoxic effects induced by exposure to fluorescent light. [Zigler, J.S.
Related QuestionsBack to top Can antibiotics and/or antimycotic agents be added to the cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: Most cell culture technologists avoid using antibiotics for routine culture work. Antibiotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks (e.g.
Related QuestionsWhat is Osmolality?
Welcome to Osmolality.com | Osmolality | FAQFollowing are frequently asked questions regarding the use of osmolality in the pharmaceutical, biotechnology, and sports medicine applications.
Related QuestionsWhy are some cell culture media better than others?
Welcome to Osmolality.com | Osmolality | FAQMedia originally used for growth of mammalian cells were based on biological fluids, such as plasma. This type of media suffered many disadvantages including batch variation and vulnerability to contamination.
Related QuestionsHow can cell culture media be made?
Welcome to Osmolality.com | Osmolality | FAQA bioreactor allows the growth of human tissues outside of the body that amass and behave like those in the body. Another is the Rotary Cell Culture System, as it provides a gentler environment than a dynamic or static tissue culture system, thereby allowing cells to aggregate, grow 3-dimensionally, and differentiate. The result will be cells or tissues that closely resemble the in vivo tissue equivalent.
Related QuestionsWhat if tissue culture medium is unavailable?
FAQ 3Samples for chromosomal analysis can still be taken. We suggest drawing maternal blood in a sterile tube, spinning it down and using the plasma or serum as transport medium for the fetal tissue samples. Studies suggest that this is considerably more successful than the use of sterile saline. If maternal serum can not be obtained, one can use sterile saline for shipment.
Related QuestionsWhat is the osmolality of Gatorade?
Frequently Asked Questions - Gatorade?In scientific terms, osmolality is a measure of the number of particles in a solution. For example, water has a very low osmolality (close to zero) where cranberry juice cocktail has a very high osmolality because there are a lot of sugar molecules dissolved in it. The osmolality of Gatorade is close to that of our blood, a factor that helps assure rapid absorption. If beverage osmolality becomes too high (e.g. soft drinks and fruit juices), the rate of fluid absorption is slowed.
Related QuestionsBack to top How do I change the medium in (feed) a suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Suspension cell lines can be fed by the simple addition of fresh medium to the culture (if room is available) or by separating the cells from the old medium by centrifugation (125 x g for 5 min) with subsequent resuspension of the cell pellet in fresh medium. However, with most suspension cell lines, a simple addition of medium is the preferred method. In either case it is absolutely necessary to feed the cultures before the cells reach their maximum saturation density.
Related QuestionsDo you sell culture medium and supplies?
FAQ From New York WormsNo. We feel that it is easier for our customers to use our started cultures rather than attempt to start their own. (See next question) Precisely the reason that we don't sell culture supplies. Unless one is extremely careful, wild "common" fruitflies can contaminate the stock, producing all flying fruitflies. The only remedy is to dispose of everything and start over.
Related QuestionsWhat should the osmolality be for growth media?
Welcome to Osmolality.com | Osmolality | FAQThe osmolality of standard cell growth media for mammalian cells is 300mOsm/kg, an optimal value for most cell lines.
Related QuestionsBack to top Why do some cell lines require sodium pyruvate? How much should I add to the medium?
ATCC: Frequently Asked QuestionsAnswer: Pyruvate is an intermediary organic acid metabolite in glycolysis and the first of the Embden Myerhoff pathway that can pass readily into or out of the cell. Thus, its addition to tissue culture medium provides both an energy source and a carbon skeleton for anabolic processes.
Related QuestionsBack to top How can I adapt a monolayer cell line to grow in suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Not all cell lines can be adapted to suspension growth. In general, normal diploid anchorage-dependent (must be attached to a substrate to grow) cells cannot be adapted without the use of microcarrier beads to which they can attach. Lines such as L-929 (ATCC CCL-1) and HeLa (ATCC CCL-2) which are not anchorage-dependent can be adapted and variants that grow in suspension already exist.
Related QuestionsBack to top What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?
ATCC: Frequently Asked QuestionsAnswer: While the levels of carbon dioxide in cell culture systems vary from that in ambient air (about 0.03%) up to 40% in air, generally either no added CO2 or 5% to 10% CO2 in air are the most frequently used. It is very important to adjust the concentration of sodium bicarbonate used in a medium to that required for equilibration with the level of CO2 used in the gas phase. Cells in culture produce CO2 and require small amounts of the compound for growth and survival.
Related QuestionsBack to top Why is it important to limit exposure of cell culture media to fluorescent lights?
ATCC: Frequently Asked QuestionsAnswer: An important but often overlooked source of chemical contamination results from the exposure of media containing riboflavin or tryptophan to normal fluorescent lighting (see references below). These media components are photoactivated by UV radiation emitted from most fluorescent lights and give rise to hydrogen peroxide. This generates free radicals that are toxic to cells; the longer the exposure the greater the toxicity.
Related QuestionsIs the glass bottom SensoPlate recommended for cell culture?
Greiner Bio OneWhile this plate provides excellent flatness and clarity for microscopic examination, some cell lines may interact with the adhesive used in production. It is best to pre-test your culture if you plan to incubate them in the SensoPlate for longer than 24 hours.
Related QuestionsWhat is the osmolality of Accelerade?
Frequently Asked Questions about Accelerade Sports Drink Pro...Osmolality is a measure of the number of particles in a solution and can affect the functionality of fluid replacement drink. The osmolality of Accelerade ranges from approximately 345 to 365 mOsm/kg. Data in the literature suggests that beverages in the range of 0 to 400 osmolality do not affect intestinal absorption or fluid homeostasis during moderately intense exercise.
Related QuestionsWhat about culture?
Center for Relationship Abuse Awareness: Frequently Asked Qu...All cultures have both traditions of resistance to domestic violence as well as forms of acceptance of it. Culture cannot excuse domestic violence—though abusers may use “culture” as a way to justify their choice to abuse. Unfortunately, relationship abuse is prevalent in all cultures—. Across the world, different cultures may have different responses to domestic violence, and some may hold abusers more accountable than others.
Related QuestionsWhat is toxicity of beads in relation to cells and cell culture?
Seradyn | Technical Support | FAQThe concern about toxicity here is that the cells can take the particles in via either phagocytosis or endocytosis (two separate cellular mechanisms for taking in particles of various sorts). Once taken in things go into endosomes and these initial membrane bounded vesicles fuse with a lysosome forming a phagolysosome. In these vesicles things get digested by various enzymes.
Related QuestionsWho uses modern cell-culture rabies vaccines?
Rabies.net - FAQCountries in North America and western Europe only use modern cell-culture rabies vaccines. The use of cell-culture rabies vaccines is increasing throughout South East Asia and the Middle East. Unfortunately, countries in Asia, Africa and Latin America, where rabies is enzootic, still rely heavily on nerve-tissue vaccines.
Related QuestionsWhy attempt to isolate mumps virus in cell culture?
NIP: Diseases/Mumps/Mumps Lab Testing Q&AsVirus isolation is considered the best method to detect infection. Virus can be detected when IgM antibodies or IgG titer rise are not detected. Additionally, it provides virus that can be used for sequence studies. Finally, isolation studies are less likely than PCR assays to give false positive results because of contamination. The sequence of the PCR product will confirm positive PCR reactions.
Related QuestionsCan 6-, 12-, 24-, or 48-well cell culture plates be used on MixMate?
Eppendorf Canada ::: FAQs-Mixers-ShakersYes, cell culture plates can be used on MixMate – as long as they comply with the SBS Standard for Microplates , and they can be mixed at a minimum mixing rate of 300 rpm.
Related QuestionsWhat is a medium?
psychic FAQ, questions about psychics and mediumsA medium is someone who has learned how to communicate with those who have passed into the world of spirit.
Related QuestionsActioNet, Inc.ActioNet has created a culture that fosters excellence amongst its management staff and employees. The company rewards its employees for their outstanding performance through our recognition, reward, and incentive plan, promotions, and the annual holiday party and picnic. ActioNet, Inc. is a young company that thrives on innovative and open thinking. Our goal is to continually strive towards process improvement. No idea is too small to be considered for implementation.Related Questions
Frequently Asked QuestionsOne meaning of "culture" is "high culture", by which we often mean the artistic tastes of a society's educated elite - for instance in New York, going to the Opera, or to the Museum of Modern Art might be considered "cultural" activities.Related Questions
How can you replace the reactions of a whole animal in a test tube or a cell culture?
Dr Hadwen Trust: faqsNon-animal research rarely simply replaces like for like. Instead we use a different approach in order to replicate the whole body scenario, replacing each type of animal experiment with a whole range of non-animal techniques that are used in combination. When it comes to studying the “whole animal” it is wrong to assume that animals are the best choice, or that they are necessary to solve every medical problem.
Related QuestionsAmazing Psychic ReadingsAlthough I have the ability to connect with those on the spiritual plane, I do not specialize in contacting those who have passed over, and prefer only to do this on rare occasions where it is necessary for healing and completion to occur, such as with the loss of a child or spouse, or in the case of suicide.Related Questions
