Back to top How can I adapt a monolayer cell line to grow in suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Not all cell lines can be adapted to suspension growth. In general, normal diploid anchorage-dependent (must be attached to a substrate to grow) cells cannot be adapted without the use of microcarrier beads to which they can attach. Lines such as L-929 (ATCC CCL-1) and HeLa (ATCC CCL-2) which are not anchorage-dependent can be adapted and variants that grow in suspension already exist.
Related QuestionsBack to top What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?
ATCC: Frequently Asked QuestionsAnswer: While the levels of carbon dioxide in cell culture systems vary from that in ambient air (about 0.03%) up to 40% in air, generally either no added CO2 or 5% to 10% CO2 in air are the most frequently used. It is very important to adjust the concentration of sodium bicarbonate used in a medium to that required for equilibration with the level of CO2 used in the gas phase. Cells in culture produce CO2 and require small amounts of the compound for growth and survival.
Related QuestionsWhy are some cell culture media better than others?
Welcome to Osmolality.com | Osmolality | FAQMedia originally used for growth of mammalian cells were based on biological fluids, such as plasma. This type of media suffered many disadvantages including batch variation and vulnerability to contamination.
Related QuestionsHow can cell culture media be made?
Welcome to Osmolality.com | Osmolality | FAQA bioreactor allows the growth of human tissues outside of the body that amass and behave like those in the body. Another is the Rotary Cell Culture System, as it provides a gentler environment than a dynamic or static tissue culture system, thereby allowing cells to aggregate, grow 3-dimensionally, and differentiate. The result will be cells or tissues that closely resemble the in vivo tissue equivalent.
Related QuestionsBack to top How do I change the medium in (feed) a suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Suspension cell lines can be fed by the simple addition of fresh medium to the culture (if room is available) or by separating the cells from the old medium by centrifugation (125 x g for 5 min) with subsequent resuspension of the cell pellet in fresh medium. However, with most suspension cell lines, a simple addition of medium is the preferred method. In either case it is absolutely necessary to feed the cultures before the cells reach their maximum saturation density.
Related QuestionsBack to top What should the osmolality of my cell culture medium be?
ATCC: Frequently Asked QuestionsAnswer: Most established vertebrate lines will tolerate a rather large variation in osmotic pressure. The useful range of osmolality of cell culture media for vertebrate lines is between 260 to 320 mOSM/kg. Invertebrate lines vary greatly in their sensitivity to osmotic pressure. For example, the snail embryo ATCC CRL-1494 requires medium of about 155 mOSM/kg, while some insect media prefer 360 to 375 mOSM/kg.
Related QuestionsHow should I subculture a monolayer?
ATCC: Frequently Asked QuestionsAnswer: Refer to the product sheet included with your ATCC cell line for the subculturing procedure recommended for that particular cell line. Anchorage-dependent cell lines are usually subcultured by disaggregation of the cell sheet with proteolytic enzymes such as trypsin. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, may be added to the dissociation solution to enhance the activity of the trypsin by removing calcium and magnesium from the surfaces of the cells.
Related QuestionsWhat is suspension?
Suspension FAQThe act of suspension is hanging the human body from (or partially from) hooks pierced through the flesh in various places around the body.
Related QuestionsBack to top Can I use HEPES buffer in my cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: HEPES and other organic buffers can be used effectively with many cell lines (see Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130: 305). However, be aware that the compound can be toxic, especially for some differentiated cell types, so its effects should be evaluated before routine use (People, C.A., et al., (1982) In Vitro 18: 755). HEPES has also been shown to greatly increase the sensitivity of media to the phototoxic effects induced by exposure to fluorescent light. [Zigler, J.S.
Related QuestionsBack to top How often should I subculture a cell line?
ATCC: Frequently Asked QuestionsAnswer: Subculture refers to the transplantation of cells from one vessel to another. The term subculture is synonymous with the terms passage and split. The subculture interval is the time between subsequent subcultures. The subculture number is simply the number of times a culture has been transferred from one vessel to another. Adherence-dependent cell lines are generally subcultured either at or near confluency.
Related QuestionsWhat is a stem cell line?
jwsoccergurly's Xanga SiteObtained for research or other uses, a stem cell line is a living colony of stem cells in a laboratory. A stem cell line comes from either a human or animal tissue and it is continuously dividing the population of cells. Even thought they deteriorate, another name for it is ?mmortal?cell lines because these lines are established from the embryos shortly after fertilization. The government will be affected by making the decision.
Related QuestionsBack to top Why is it important to limit exposure of cell culture media to fluorescent lights?
ATCC: Frequently Asked QuestionsAnswer: An important but often overlooked source of chemical contamination results from the exposure of media containing riboflavin or tryptophan to normal fluorescent lighting (see references below). These media components are photoactivated by UV radiation emitted from most fluorescent lights and give rise to hydrogen peroxide. This generates free radicals that are toxic to cells; the longer the exposure the greater the toxicity.
Related QuestionsBack to top Can antibiotics and/or antimycotic agents be added to the cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: Most cell culture technologists avoid using antibiotics for routine culture work. Antibiotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks (e.g.
Related QuestionsIs the glass bottom SensoPlate recommended for cell culture?
Greiner Bio OneWhile this plate provides excellent flatness and clarity for microscopic examination, some cell lines may interact with the adhesive used in production. It is best to pre-test your culture if you plan to incubate them in the SensoPlate for longer than 24 hours.
Related QuestionsHow many students currently have land-line service, as opposed to cell phones?
Stanford Residential Computing: For Students: Services: In-R...In 2004-05, as of Winter quarter, 38% of undergraduate rooms and 60% of graduate rooms on campus had land-line service, or about 50% of all rooms on campus. Since a majority of students live in doubles, triples, or quads, the number of students, as opposed to the number of rooms, served by land-line telephones is higher than that. We estimate that 50% of undergraduates and 75% of graduate students on campus have land-lines in their rooms.
Related QuestionsWhy would someone want to do a suspension?
Suspension FAQThere are many different reasons to suspend, from pure adrenaline or endorphin rush, to conquering ones fears, to trying to reach a new level spiritual consciousness and everything in between. In general, people suspend to attain some sort of "experience". Some people are seeking the opportunity to discover a deeper sense of themself and to challenge pre-determined belief systems which may not be true.
Related QuestionsHow do they adapt?
Frequently Asked QuestionsGreyhounds love people, in fact more than most breeds, and tend to be quite sociable. They have been handled a great deal during their early years by dog walkers, trainers, veterinarians and others. Many trainers are women who bring their children to work, so the dogs frequently have been exposed to children of all ages.
Related QuestionsBack to top How do I grow hybridoma cell lines as ascites, especially rat-mouse hybridomas?
ATCC: Frequently Asked QuestionsAnswer: ATCC propagates most hybridomas as cell cultures in vitro, but many can be grown efficiently as ascites by inoculation into the peritoneal cavities of mice. Some rat-mouse hybridomas are more problematical as the host mouse strain may develop anti-rat antibodies and eventually reject the hybridoma inoculum. To minimize this difficulty one can use athymic nude mice as hosts or immunosuppress host animals prior to inoculation. For a protocol see p.
Related QuestionsBack to top Can a cell line be cured of mycoplasma contamination?
ATCC: Frequently Asked QuestionsAnswer: Yes, several lines in our collection have been cured of mycoplasma (e.g., ATCC CCL-229, ATCC HB-175). However, this process is time consuming and does not always work; discarding the culture and starting over is always the preferred method. As with other microbial infections, one should first identify the contaminant and select a suitable antibiotic, preferably by testing the contaminating mycoplasma for its antibiotic sensitivity.
Related QuestionsBack to top What are some factors that I must consider when depositing my cell line at ATCC?
ATCC: Frequently Asked QuestionsAnswer: Some factors to consider before submitting a new line are: 1) the cells should exhibit some unique characteristics or uses not exhibited by cells already in the collection; 2) the cell line should be documented in literature; 3) the cells must have a "useful" life expectancy in vitro, that is at least 15 to 20 doublings beyond the culture supplied; and 4) the cells should be free of contaminating microorganisms.
Related QuestionsWho owns my DNA cell line after it has been preserved?
Perpetuate - Pet DNA and Cell BankingYou maintain complete ownership of your pet’s cell line after it has been developed and preserved.
Related QuestionsCan a cell line be developed from any cellular material other than tissue?
Perpetuate - Pet DNA and Cell BankingNo, only viable cells from animal tissue can be used to clone an animal. Animal blood, fur, teeth, bones, nails and so forth can not be used.
Related QuestionsMichigan Citizens for Stem Cell Research & CuresEmbryonic stem cells are isolated by transferring the inner cell mass into a plastic laboratory culture dish. Growing embryonic stem cells in the laboratory is known as cell culture. The cells divide and spread over the surface of the culture dish, which is coated with connective tissue cells that have been treated so they will not divide. After stem cells replicate for several days, they crowd the dish. They are removed gently and replated into several fresh culture dishes.Related Questions
What are the best selective culture media to grow Lactobacillus?
LISTSERV General User's GuideThe simplest way to look for a list is to search the so-called "list of lists" that LISTSERV maintains automatically. Now, it would probably be unrealistic to expect to find a list dedicated to the various kinds of selective culture media used to grow Lactobacillus. Even with nearly 10,000 public lists, that would still leave us with a couple dozen lists on various aspects of lactobacilli, and not very much space left for the computer experts to talk about virtual reality and C++.
Related QuestionsWhat about culture?
Center for Relationship Abuse Awareness: Frequently Asked Qu...All cultures have both traditions of resistance to domestic violence as well as forms of acceptance of it. Culture cannot excuse domestic violence—though abusers may use “culture” as a way to justify their choice to abuse. Unfortunately, relationship abuse is prevalent in all cultures—. Across the world, different cultures may have different responses to domestic violence, and some may hold abusers more accountable than others.
Related QuestionsHow can I adapt my Harrick liquid cell, with Luer fittings, for flow-through applications?
Harrick - Frequently Asked QuestionsSeveral Harrick liquid accessories and attachments have female Luer fittings. Such fittings are ideal for the injection of discrete samples. For convenient use in flow-through applications, adapters are readily available from Upchurch Scientific, Inc. (P.O. Box 1529; 619 Oak Street; Oak Harbor, WA 98277; 800-426-0191; FAX: 800-359-3460; www.upchurch.com). Note: These Upchurch parts, discussed further below, are not suitable for high-pressure applications.
Related Questions