Why attempt to isolate mumps virus in cell culture?
NIP: Diseases/Mumps/Mumps Lab Testing Q&AsVirus isolation is considered the best method to detect infection. Virus can be detected when IgM antibodies or IgG titer rise are not detected. Additionally, it provides virus that can be used for sequence studies. Finally, isolation studies are less likely than PCR assays to give false positive results because of contamination. The sequence of the PCR product will confirm positive PCR reactions.
Related QuestionsWhy are some cell culture media better than others?
Welcome to Osmolality.com | Osmolality | FAQMedia originally used for growth of mammalian cells were based on biological fluids, such as plasma. This type of media suffered many disadvantages including batch variation and vulnerability to contamination.
Related QuestionsHow can cell culture media be made?
Welcome to Osmolality.com | Osmolality | FAQA bioreactor allows the growth of human tissues outside of the body that amass and behave like those in the body. Another is the Rotary Cell Culture System, as it provides a gentler environment than a dynamic or static tissue culture system, thereby allowing cells to aggregate, grow 3-dimensionally, and differentiate. The result will be cells or tissues that closely resemble the in vivo tissue equivalent.
Related QuestionsIs the strain of mumps virus in Iowa a rare strain?
Frequently Asked QuestionsNo, it is the same strain seen in an outbreak in England. The current MMR vaccine covers this strain. No. There is no correlation to disease and vaccination at 12 months of age. Infants used to be routinely vaccinated at 15 months of age. During the 1989-1991 measles resurgence, incidence rates of measles were more than twice as high as those in other age groups.
Related QuestionsHow is the mumps virus spread?
Mumps - Frequently Asked QuestionsMumps is spread by contact with discharges from the nose and throat. Coughing, sneezing, sharing drinks and kissing can spread these discharges. The virus can be spread from about seven (7) days before glands become swollen, to nine (9) days after. A person who is infected with mumps virus but does not have any symptoms can still spread the virus to other people
Related QuestionsHow is the mumps virus transmitted?
Mumps FAQThe mumps virus replicates in the upper respiratory tract and is spread through direct contact with respiratory secretions or saliva or through fomites.
Related QuestionsBack to top What should the osmolality of my cell culture medium be?
ATCC: Frequently Asked QuestionsAnswer: Most established vertebrate lines will tolerate a rather large variation in osmotic pressure. The useful range of osmolality of cell culture media for vertebrate lines is between 260 to 320 mOSM/kg. Invertebrate lines vary greatly in their sensitivity to osmotic pressure. For example, the snail embryo ATCC CRL-1494 requires medium of about 155 mOSM/kg, while some insect media prefer 360 to 375 mOSM/kg.
Related QuestionsIs the strain of mumps virus in Iowa and the Midwest a rare strain?
Mumps FAQNo, it is the same strain seen in an outbreak in England, and that has also been identified in Canada, Croatia, and Nepal. Source: National Immunization Program web site: http://www.cdc.gov/nip/diseases/mumps/mumps-tech-faqs.htm File Formats | Oregon Administrative Rules | Oregon Revised Statutes | Privacy Policy | Web Site Feedback|
Related QuestionsBack to top Can I use HEPES buffer in my cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: HEPES and other organic buffers can be used effectively with many cell lines (see Shipman, C. (1969) Proc. Soc. Exp. Biol. Med. 130: 305). However, be aware that the compound can be toxic, especially for some differentiated cell types, so its effects should be evaluated before routine use (People, C.A., et al., (1982) In Vitro 18: 755). HEPES has also been shown to greatly increase the sensitivity of media to the phototoxic effects induced by exposure to fluorescent light. [Zigler, J.S.
Related QuestionsWho can get mumps?
Vaccines: VPD-VAC/Mumps/Disease FAQsAnyone who is not immune from either previous mumps infection or from vaccination can get mumps. Before the routine vaccination program was introduced in the United States, mumps was a common illness in infants, children and young adults. Because most people have now been vaccinated, mumps is now a rare disease in the United States. Of those people who do get mumps, up to half have very mild, or no symptoms, and therefore do not know they were infected with mumps.
Related QuestionsQuality Health | MumpsMumps is a contagious viral infection that can cause painful swelling of the parotid glands , which are the salivary glands located between the ear and the jaw.Related Questions
Mumps - Frequently Asked QuestionsIt is spread by direct contact with discharge from the nose and throat from 7 days before the onset of swollen glands 9 days after. Some people can be infected and have no symptoms. Most people recover completely from the illness but mumps can be serious. Those who become very ill may have meningitis (a swelling of the covering of spinal cord and the brain). If these symptoms occur, seek medical care immediately. One in three men may get painful, swollen testicles.Related Questions
Back to top How can I adapt a monolayer cell line to grow in suspension culture?
ATCC: Frequently Asked QuestionsAnswer: Not all cell lines can be adapted to suspension growth. In general, normal diploid anchorage-dependent (must be attached to a substrate to grow) cells cannot be adapted without the use of microcarrier beads to which they can attach. Lines such as L-929 (ATCC CCL-1) and HeLa (ATCC CCL-2) which are not anchorage-dependent can be adapted and variants that grow in suspension already exist.
Related QuestionsBack to top What are the recommended carbon dioxide (CO2) levels needed to grow a cell culture?
ATCC: Frequently Asked QuestionsAnswer: While the levels of carbon dioxide in cell culture systems vary from that in ambient air (about 0.03%) up to 40% in air, generally either no added CO2 or 5% to 10% CO2 in air are the most frequently used. It is very important to adjust the concentration of sodium bicarbonate used in a medium to that required for equilibration with the level of CO2 used in the gas phase. Cells in culture produce CO2 and require small amounts of the compound for growth and survival.
Related QuestionsBack to top Why is it important to limit exposure of cell culture media to fluorescent lights?
ATCC: Frequently Asked QuestionsAnswer: An important but often overlooked source of chemical contamination results from the exposure of media containing riboflavin or tryptophan to normal fluorescent lighting (see references below). These media components are photoactivated by UV radiation emitted from most fluorescent lights and give rise to hydrogen peroxide. This generates free radicals that are toxic to cells; the longer the exposure the greater the toxicity.
Related QuestionsBack to top Can antibiotics and/or antimycotic agents be added to the cell culture medium?
ATCC: Frequently Asked QuestionsAnswer: Most cell culture technologists avoid using antibiotics for routine culture work. Antibiotics may mask contamination by susceptible bacteria and fungi while permitting mycoplasma to flourish unnoticed. Antibiotics may interfere with the metabolism of sensitive cells in culture. However, one may elect to introduce antibiotics for short periods to primary cultures or as a safeguard while propagating specific valuable stocks (e.g.
Related QuestionsIs the glass bottom SensoPlate recommended for cell culture?
Greiner Bio OneWhile this plate provides excellent flatness and clarity for microscopic examination, some cell lines may interact with the adhesive used in production. It is best to pre-test your culture if you plan to incubate them in the SensoPlate for longer than 24 hours.
Related QuestionsDoes the current vaccine work against the mumps virus that is causing the outbreak?
NIP: Diseases/Mumps/Outbreak FAQsYes. The strain of mumps virus in the Midwest is the same as the one that is found in other countries, and that caused a large ongoing outbreak in the United Kingdom (UK) with more than 60,000 cases. In 2005 a small mumps outbreak occurred in the US after a person visited from the UK and mumps vaccine was effective in controlling this outbreak.
Related QuestionsWhat is whey protein isolate?
BiPro Whey Protein Isolate - frequently asked questionsAccording to the Food and Drug Administration (FDA) whey protein isolate is a natural dairy protein powder made up of at least 90% protein. BiPro whey protein isolate contains 20 grams protein/22 gram serving. As a protein source, whey protein isolate is a better choice than whey protein concentrate, which contains about 80% protein. Also, whey protein isolate contains almost no sugar (lactose) or fat.
Related QuestionsWhat about culture?
Center for Relationship Abuse Awareness: Frequently Asked Qu...All cultures have both traditions of resistance to domestic violence as well as forms of acceptance of it. Culture cannot excuse domestic violence—though abusers may use “culture” as a way to justify their choice to abuse. Unfortunately, relationship abuse is prevalent in all cultures—. Across the world, different cultures may have different responses to domestic violence, and some may hold abusers more accountable than others.
Related QuestionsHow does a virus attack a cell?
CurEvents.com - A Global Current Events Discussion Forum - S...Imagine a pirate ship with grappling hooks. The virus will throw a bunch of grappling hooks at a cell and bind to its surface. Then it will attack the cell, by working its way through the cell membrane. Once it's inside the cell, it'll make its way to the nucleus. The virus then orders the cell's machinery (every cell essentially is like a little factory) to make new virus particles. A single cell will produce between 100,000 and a million influenza virus particles.
Related QuestionsWhat is toxicity of beads in relation to cells and cell culture?
Seradyn | Technical Support | FAQThe concern about toxicity here is that the cells can take the particles in via either phagocytosis or endocytosis (two separate cellular mechanisms for taking in particles of various sorts). Once taken in things go into endosomes and these initial membrane bounded vesicles fuse with a lysosome forming a phagolysosome. In these vesicles things get digested by various enzymes.
Related QuestionsWho uses modern cell-culture rabies vaccines?
Rabies.net - FAQCountries in North America and western Europe only use modern cell-culture rabies vaccines. The use of cell-culture rabies vaccines is increasing throughout South East Asia and the Middle East. Unfortunately, countries in Asia, Africa and Latin America, where rabies is enzootic, still rely heavily on nerve-tissue vaccines.
Related QuestionsCan 6-, 12-, 24-, or 48-well cell culture plates be used on MixMate?
Eppendorf Canada ::: FAQs-Mixers-ShakersYes, cell culture plates can be used on MixMate – as long as they comply with the SBS Standard for Microplates , and they can be mixed at a minimum mixing rate of 300 rpm.
Related QuestionsWhat constitutes an exposure for mumps?
Frequently Asked QuestionsThe potential for droplet (within three feet) contamination from nasal or oral secretions, or direct contact with saliva, when the case was in the infectious period and without the use of appropriate PPE - gloves and mask, would be considered exposed. There is not a specific length of time. Mumps is droplet spread and contagiousness is similar to influenza and rubella, less than measles and varicella.
Related QuestionsHave any complications from the mumps been reported?
Frequently Asked Questionsof 3/23/06, we have had 5/69 (7 percent) reported cases of orchitis and one possible case of encephalitis. Watch the" Iowa Mumps Update" for current information, and the update is posted weekly on the IDPH web site.
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