If you want to do a two-hybrid or similar screen start with the Quick and Easy Transformation Procedure to transform your bait plasmid into the yeast strain you will be using. Then use the Standard Transformation procedure to do an experiment to test your library plasmid DNA for transformation yield to determine the scale you wish to use. Finally, use the High Efficiency Library Transformation procedure with the appropriate scale to generate the transformants to screen your library. FAQ

The palm Tungsten T3 has a retractable "virtual" graffiti area that allows applications to use the full 320x480 pixel screen. The Palm OS standard for accessing this area was still being defined when the T3 shipped, so palm included non-standard support for this feature. A Tungsten T3 right out of the box doesn't have full, standard support for the retractable graffiti area. See similar questions...

Fetchmail can use RFC1731 GSSAPI authorization to safely identify you to your IMAP server, as long as you can share Kerberos V credentials with your mail host and you have a GSSAPI-capable IMAP server. UW-IMAP (available via FTP at ftp.cac.washington.edu) is the only one I'm aware of and the one I recommend anyway for other reasons. You'll need version 4.1-FINAL or greater though, and it has to have GSS support compiled in. See similar questions...

It can be very difficult to determine what constitutes human subjects research and when to file a human subjects protocol. 45 CFR 46 offers the following definitions as guidance for determining human subjects research. The definitions are intentionally broad to include a wide range of research in hopes of capturing both the biomedical and humanities spectrums. See similar questions...

Note: If you are not familiar with changing network settings, please visit Section 6 of Internet Texoma's How To directory on this site. A When removing network protocols, the protocol that will be used (TCP/IP) must be installed before removing the unneeded protocols. Note: If you are not familiar with changing network settings, please visit Section 6 of Internet Texoma's How To directory on this site. See similar questions...

There is an RFC1123 requirement that MAIL FROM and RCPT TO addresses you pass to it have to be canonical (e.g. with a fully qualified hostname part). Therefore fetchmail tries to pass fully qualified RCPT TO addresses. But exim does not by default accept 'localhost' as a fully qualified domain. This can be fixed. In exim.conf, add 'localhost' to your local_domains declaration if it's not already present. For example, the author's site at thyrsus. See similar questions...

Yes. You need to answer each question in order to submit the Animal Use Protocol. If you do not answer every question you not be allowed to submit the Protocol. If an answer is incomplete, your Animal Use Protocol may be delayed and you will receive an email from the IACUC asking for more information. See similar questions...

Note: If you are not familiar with changing network settings, please visit Section 6 of Internet Texoma's How To directory on this site. See similar questions...

Definitely not. While protocol analyzers can be used by network developers to view the exact contents of a network conversation, a modern protocol analyzer with a graphical user interface provides many other types of information beyond the bits and bytes of the actual protocols. Being able to see which device or system failed to respond is usually enough information to pinpoint the problem and focus your attention on that piece of the puzzle. See similar questions...

While most actions of thyroid hormone are most likely due to T3, most T3 in the body comes from the conversion of T4. The conversion of T4 to T3 is normal in hypothyroid patients, so the body can regulate to levels of T4 just as if the T4 was being produced from the thyroid gland. T3 has a very short life span in the body, while the life span of T4 is much longer, ensuring a steady supply of T3. There is no indication for the use of T3 alone for the treatment of hypothyroidism. See similar questions...

The ACT-R site at CMU maintins a list of models that are available online. It also has a list of models referenced in published papers. Some of these models are availble upon request. See similar questions...

Wear the T3 around your neck with the 1/2" bolt snap fastened in the small loop. The whistle is attached to a small snap swivel at the bottom of the handloop. To convert the T3 from a whistle lanyard to a leash, unfasten the bolt snap (with one hand) and attach it to a dog's collar. The resulting 32" leash is convenient and tough enough to control your dog. The whistle on the handloop is out of the way at the heel of your hand. See similar questions...

You can, but you should not expect the rates of infection and mortality to be normalized to that of the nondiabetic population if the protocol is not utilized until the morning of the third postoperative day. It will be reduced for sure, but one will not affect the complete dramatic results we have seen. There is both a physiologic and clinical basis for the rationale of running the drip until the third postoperative day. See similar questions...

Wowza Media Server communicates with the Adobe Flash Player client using RTMP, RTMPT and RTMPS. Your choice of the RTMP variant depends upon your application needs. See similar questions...

Many of our products come with use instructions, and almost all of our products come with a list of related articles. Even when we have no specific information, however, we may be able to direct you to references or protocols for related products. Please send your questions by fax: 7 (495) 330 55 92 or e-mail: atanov@carb.ibch.ru or carb@lectinity.com See similar questions...

No, you don't have to but we recommend you implement some way to find out if the data sent was received by the receiver and contains valid data. There is many ways to do this and our tip is to start simple and go See similar questions...

Once the extensions have been activated, using the FTP protocol would jeopardise correct publication. You would probably risk jeopardising the extensions on the server. For instance: if the registered domain is mariorossi.it the correct form to use for publication is http://www.mariorossi.it See similar questions...

VaxVoice SDKs use UDP protocol for voice streaming. Because, UDP protocol is a preferred protocol to send voice data over the IP based Network. See similar questions...