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Frequently Asked Questions

Can you inject a polyacrylamide gel? Nitrocellulose?

Pocono Rabbit Farm and Laboratory Inc. - PRF&L
Yes, we can inject a gel slice. Run the antigen on the gel, stain with Coomassie Blue, cut out the band of interest and place in a tube with just enough distilled water or PBS to keep the gel slice moist. Coomassie Blue is not toxic to the animals. Try not to use a protocol that uses much ascetic acid. Please do not freeze the gel prep. Antigens in gel are stable at 4 degrees Celsius. You may send the gel at room temperature or with an ice pack. Estimate how much you are sending.

Why won't my polyacrylamide gel polymerize?

Methods FAQ list
Poor or slow polymerization of acrylamide is most likely the result of an older solution of ammonium persulfate (APS) solution which has gone off. First try making a fresh stock from solid material. Netters have several quick tests to see if the APS is still good. One way is to put some in a test tube and add the correct volume of water to make 10 mg/ml. If you hear it snap, crackle, and pop, then it is still good.

What is nitrocellulose?

Answers to Frequently Asked Questions
Nitrocellulose is an ester of cellulose and nitric acid. It is used as a component of many lacquers, inks, adhesives and cements. It becomes very unstable when heated and dry. It auto ignites at 275 F - 330 F in a violent , exothermic reaction to form a dense cloud containing potentially toxic levels of nitrogen oxides and carbon monoxide. It is extremely corrosive. Do not process nitrocellulose in your Sidewinder. It is dangerous and related damage is not covered by warranty.

What is the difference between Nitrocellulose Lacquer and Polyurothane?

Frequently Asked Questions
Nitro is often called a "thin skin" as it really allows the guitar to resonate freely. It has a superior feel and a super glossy look to it. Poly is almost like a thin layer of plastic. It feels tacky and doesn't allow the guitar to resonate very well. Most companies use poly because its faster to work with.

Where do I inject myself?

Human Growth Hormone FAQs and HGH Side Effects
You can inject it sub-cutaneously (just under the skin) in the stomach area or leg with an 29 - 30 gauge, 1/2 inch, 1cc insulin syringe, or alternatively you can inject it intramuscular, however the results from sub-cutaneous injections are more effective.

Where do I inject?

Q&A about IGF-1 ::
Some say that intramuscular is best, but it shouldn't really matter. You can inject it into the stomach fat (like insulin) or into the muscle.

When should I inject the back-up dose?

FAQ's about allergies and anaphylaxis (Twinject)
Prepare the back-up dose immediately and call 911 or contact a medical facility. Inject the back-up dose if the symptoms have not subsided within 10 minutes of the first dose or have recurred.

Where do I inject my fertility medications?

FertilityMeds.com/Frequently Asked Questions
The new generation of recombinant gonadotropins, including Gonal-F, Follistim, and Ovidrel, may be given subcutaneously as the preferred route of administration. The older generation of gonadotropin preparations, such as Pergonal, was approved for intramuscular administration. Repronex has been approved for both intramuscular and subcutaneous injection. hCG has been approved for intramuscular injection in the past.

Why is it called a gel?

Stardust - Frequently Asked Questions
During the production of aerogel a wet gel is formed which when dried becomes filled with air. Thus the name aerogel, which means: air gel.

What about a nitrocellulose lacquer finish? Can I get that on my bass?

LeCompte Electric Bass - Custom Bass Guitars
While I can't guarantee all those things, I will do my best at making recommendations and steer you in the right direction if you need some help picking out parts and materials. Keep in mind that what we build are custom basses and the bass you order will end up sounding how it ends up sounding. I use the best materials I can find. If you're putting the good stuff into a bass build it only makes sense that bass will end up sounding great.

How many embryos do you inject?

faq
We prefer to inject 30-40 embryos per clone. If we inject more than one clone a day we average around 60-70 embryos a day.

How do you inject the fuel?

Angel Labs :: Technical
We are planning to use both a current and more advanced proprietary fuel injector, invented by Raphial. We believe the advanced injector is a cost effective design and that this new fuel injector will eliminate pre-ignition, detonation and knock events, responsible for the annoying marble sound inherent to Diesel Engines.

I don't have any spray equipment. Do I *have* to use nitrocellulose lacquer on my instrument?

Musical Instrument Makers Forum miniFAQ: Acoustic Guitar Mak...
No, you do not. It was Bob Taylor who said, "A long time ago, some guy figured out that if you keep spraying and sanding a guitar, you can make it really shiny. We need to exhume that guy and shoot him!" While nitrocellulose lacquer has become "traditional" and a mirror-like lacquer finish is the standard, there are many other finish options including waterbased/waterborne lacquers, French polish/shellac, varnish, and various oil-based finishes like Tru Oil.

How many places can I inject and where?

EliteFitness.com - Underground Bodybuilding, Fitness and Ste...
Any muscle in your body! Glutes, ti's, bi's, lats, traps, delts, pecs, quads, calfs (but these tend to hurt like a bitch), ETC.

Where/how do I inject insulin?

Basskilleronline.com - Acnemans Insulin FAQ
The best sites for insulin injection are in the subcutaneous tissue of the abdomen (avoid the area close to bellybutton) .Usually, you should not inject within 1 inch of the same site within 1 month. The arms and legs can also be used, but insulin uptake from these sites is less uniform. Insulin should be injected subcutaneously only with a U-100 insulin syringe. "B-D ultra-fine" insulin syringes are good. Insulin syringes are available without a prescription in many states.
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